Impact of pyridoxin (B6) on gene expression of ica A and icaC Of Staphylococcus aureus bacteria

Abstract

Pathogenic bacteria Staphylococcus aureusmostly forms biofilms by adhering themselves on both tissues and inorganic surfaces by produced polysaccharide matrix as a survival mechanism in harsh environment. Which make themunreachable to biocides, antibiotics, or host immune system. This led us to seek analternative to antibiotics that inhibitformation of biofilm, so in this studytestedthe impact of pyridoxine (vitamin B6) on the expression of icaA and icaC genes which isimportant for biofilm formation in Staphylococcus aureususing qRT-PCR. The pyridoxinewas effective against biofilm-embedded S. aureus at sub inhibitory concentrations range from 6.4 μg/mL to12.5 μg/ml.Expression levels of both icaA and icaC were reduced in 85% of S. aureus adhering strain. While increase in 15% of S. aureus adhering strain. Theresearch showed a novel mechanism of pyridoxine's action, that involved in inhibition of S. aureus adhesion by reducing the expression of both icaA and icaC genes associated with staphylococcal biofilmformation.