Comparative gene expression analysis of lactate dehydrogenase isoforms in rats treated with Baba

Abstract

This study was done to observe the effect of β-amino butyric acid (BABA) on the gene expression of five Lactate dehydrogenase isomers, which is considered one of the liver function proteins (LDH). That is used to convert pyruvate into lactate and vice versa, and the importance of inhibiting it in the presence of cancer cells because it enhances their activity. We also studied the effect of BABA on the enzyme Gamma-glutamyltranspeptidase (GGT), which plays a role in glutathione metabolism and the recovery of the amino acid cysteine. Sprague-Dawley rats male were used in this study (25 rats age 2 - 3 months and weights (208-152). Rats were divided into 5 groups (five rats each) according to the concentration of BABA as follows: Control group (A) was injected with normal saline; Group B animals were injected with a concentration of 100 mg/kg; Group C was injected at a concentration of 150 mg/kg; Group D was injected at a concentration of 200 mg/kg; Group E was injected at a concentration of 250 mg/kg. It was injected six times within three weeks. Later, blood was drawn and serum was obtained to conduct the necessary tests for LDH and GGT showed that there were significant differences at the level of probability (0.05), and the effects of the amino acid BABA were confirmed as activating or inhibiting the level of enzymes. For the LDH isoenzymes, higher levels were found in treatment E increased the gene expression levels of the five isoenzymes: LDA-1, LDA-2, LDH, LDB, LDC. This is undesirable because high levels of this enzyme indicate increased activity of cancer cells, while in the other treatments the level of the enzyme decreased compared to control samples On the other hand, the GGT appeared at the highest concentration in treatment D this treatment did not activate the enzyme, but rather it remained within its normal level represented by the control treatment as for the lowest level only during treatments B, C, and E. This may cause a defect in the processes of oxidation and reduction of glutathione and difficulty in recovering the amino acid cysteine