Study extraction and antifungal effect of oat Beta-glucan against Aspergillusflavus in mice

Abstract

Objective: In Iraq, may be no research or information about the use of β-glucan extracted from oat as an antifungal against Aspergillusflavus. Therefore, the aim of this study is to evaluate the effect of oat β-glucan against A. flavusin mice.

Materials and Methods:Fifty nasal swabs were collected from dogs (stray and veterinary clinics) suffering from respiratory. Forty of albino mice used for the experiment and divided into four groups, each group contained 10 mice. Group1 was a control (un-treated), group2 control positive for Aspergillus flavusat dose (1 ×10 ⁶) spore/ mlintraperitonealI/P for 2 weeks, in group3, the mice treated with oat β-glucan in concentration 500µg in 100 µl per mouse for ten days and group4 was served as a control oat β-glucanintraperitoneal injection in same dosage of group 3;at the end of experimenthematology and histopathology examination.

Results:The results of isolation were reported a high percentage of Aspergillusspp. at 42%, which included (A. flavus andA. niger at 18% respectively, and A. terreus at 6%). The results of oat β-glucan extraction by hot water method were 3 grams from 100grams of oat. The HPLC analysis revealed its structural similarity to the standard of oat β-glucan. The hematological study shows alteration in blood parameters after infection by A. flavus and treated by oat β-glucancause significantly increase (P≤0.01)in blood parameters(WBCs, neutrophil, lymphocyte and monocytes)and significant decrease (P≤0.05)in RBCsand Hb. Histopathological study findings demonstrated different degrees of inflammation in three organs (lung, liver and kidney) in second group included presence of hyphae, necrosis, amyloid deposition and degeneration while in third group that included treatment by oat β-glucan was better therapy.

Conclusion: The present study was concluded that the A.flavus causes high pathological lesions and the oat β-glucanhas therapeutics activity against A.flavusdepending on the hematological and histopathological findings.