Chlorella Vulgaris has a Protective Effect against Gibberellic Acid-induced Lung Injury in Male Albino Rats

Abstract

Background:Gibberellic acid (GA3) is a plant growth regulator used extensively in many nations that affects the health of humans and animals.

Objectives: This study aimed to assess the potential harm of GA3 on the lung and investigated the potential protection of Chlorella vulgaris (ChV) against the GA3-induced structural and morphometric alterations in the lung.

Design:An animal experimental trial.

Setting:

Materials & methods:we enrolledadult male albino rats which were divided into 4 groups: a negative control group (G1), ChV-group (G2)treated with ChV (300mg/kg body weight once daily for 14 days), a GA3-treated group (G3)treated with GA3 (55mg/Kg once daily for 14 days), and GA3/ChV-treated group (G4)concomitantly treated with ChV and GA3 with the same dose and duration. Lung specimens were processed for microscopic assessment, biochemical study, morphometric analysis, and an immunohistochemical study.

Sample size: twenty-four adult male albino rats were included in our study.

Results: Thickened interalveolar septum with inflammatory cellular infiltration and congested thick wall blood vessels were observed in the GA3-treated group. A high-gradeincrease in the area percent of collagen fibers, the mean thickness of the inter-alveolar septa, and a considerable decrease in the mean alveolar space surface area were detected in the grouptreated with GA3-treated groupcompared to the negative control group. An immunohistochemical study showed a strong positive cytoplasmic reaction of Caspase-3 in the GA3-treated group. A high concentration of TNF-αwas noted in G3 compared to the G1.

Conclusion:The histological, morphometrical, and biochemical alterations brought on by Gibberellic acid in the lung could be ameliorated by Chlorella vulgaris.

Limitations:relatively small sample size and short study duration.

Conflict of interest: no conflicts of interest