Detection of Mycoplasma gallisepticum in fertile eggs of chickens by culture and PCR

Abstract

This study aimed to assess the occurrence of Mycoplasma gallisepticum (MG) infections in fertile eggs using culture and molecular detection. 62 egg yolk samples were collected randomly from August 2022 to April 2023 and cultured on PPLO media for mycoplasma. By using the cultural approach, the rate of incidence of Mycoplasma was 51% (32/62)13\62 (20%) positive samples found in local fertile eggs while19\62 (30%) found in imported fertile eggs,19\62 (30%), we confirmed this result by (PCR) usingspecific primers targeting16SrRNA.The PCR 330bp product was sent to the MACROGEM(Korea)for sequencing, then submitted tothe Gene bank database with accession number: OR784572.1. Sequencing alignment showed that local (MG) isolates were highly identical with standard references at the gene bank, based on the nucleotide phylogenetic tree of the 16SrRNA gene we found that the Iraqi isolate was 100%similar to the USA, South Africa, United Kingdom Iraq: Baghdad Russia Australia isolates. Also, it was 99% identical to Japan, Iraq: Basrah, Urbana, Egypt, and India. This study confirms the vertical transmission of (MG) from hens to their fertile eggs Iraqi strains have high identical similarity, to the global strains.